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An enzyme-linked immunosorbent assay (ELISA), using an autoclave-extracted soluble antigen, for the detection of serum antibodies to Brucella ovis in sheep was developed. The test seemed to be both sensitive and specific, on the basis of the control groups studied. The antigen showed no deterioration in prepared plates stored at -70 C for up to a year. The ELISA was used in conjunction with palpation of rams for epididymal lesions as a means to detect and control B ovis infection in a naturally infected flock. All rams were evaluated by the ELISA. At the time that the first blood sample was obtained, all positive and suspicious reactors were removed. With subsequent blood sample collections, only the positive reactors were removed. Brucella ovis was not isolated from any rams during the following year, and none of the mature breeding rams developed epididymal lesions.
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
July 1987,
Research in veterinary science,
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
November 1983,
The Veterinary record,
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
September 1987,
Research in veterinary science,
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
March 1984,
Research in veterinary science,
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
March 1996,
The veterinary quarterly,
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
August 1985,
Australian veterinary journal,
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
February 2005,
Journal of clinical microbiology,
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
February 1998,
Journal of medical microbiology,
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
January 1983,
The British veterinary journal,
R L Walker, and
B R LeaMaster, and
J N Stellflug, and
E L Biberstein
April 2006,
Clinical and vaccine immunology : CVI,
