BIOMAT Database Logo BIOMAT Database Logo

Changes in erythrocyte transketolase activity and the thiamine pyrophosphate effect during storage of blood. 1985

J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray

Whole blood and washed erythrocytes from nutritionally normal and abnormal elderly patients were stored at room temperature, 4 degrees C and -20 degrees C for up to 14 days. In both groups, and under all storage conditions, the erythrocyte transketolase (ETK) activity and the thiamine pyrophosphate (TPP) effect decreased with time of storage. The ETK activity for specimens stored at -20 degrees C decreased significantly by day 14 (P less than 0.05). The TPP effect in specimens stored at -20 degrees C decreased significantly in the nutritionally abnormal group at 4 days (P less than 0.05) and in the nutritionally normal group by day 14 (P less than 0.05). These significant changes in ETK activity and TPP effect with storage can lead to misleading interpretation of thiamine nutritional status.

UI MeSH Term Description Entries
D009748 Nutrition Disorders Disorders caused by nutritional imbalance, either overnutrition or undernutrition. Nutritional Disorders,Nutrition Disorder,Nutritional Disorder
D004355 Drug Stability The chemical and physical integrity of a pharmaceutical product. Drug Shelf Life,Drugs Shelf Lives,Shelf Life, Drugs,Drug Stabilities,Drugs Shelf Life,Drugs Shelf Live,Life, Drugs Shelf,Shelf Life, Drug,Shelf Live, Drugs,Shelf Lives, Drugs
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D005188 False Negative Reactions Negative test results in subjects who possess the attribute for which the test is conducted. The labeling of diseased persons as healthy when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed) False Negative Reaction,Reaction, False Negative,Reactions, False Negative
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000368 Aged A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available. Elderly
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D013835 Thiamine Pyrophosphate The coenzyme form of Vitamin B1 present in many animal tissues. It is a required intermediate in the PYRUVATE DEHYDROGENASE COMPLEX and the KETOGLUTARATE DEHYDROGENASE COMPLEX. Cocarboxylase,Thiamine Diphosphate,Berolase,Pyrophosphate, Thiamine
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D014174 Transketolase An enzyme of the transferase class that catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate in the PENTOSE PHOSPHATE PATHWAY. (Dorland, 27th ed) EC 2.2.1.1. Apotransketolase,Glycolaldehydetransferase

Related Publications

J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
Thiamine pyrophosphate effect and erythrocyte transketolase activity during severe alcohol withdrawal syndrome.
August 1993, Acta psychiatrica Scandinavica,
J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
Assessment of the thiamine nutritional status. An evaluation of erythrocyte transketolase activity, the stimulated erythrocyte transketolase activity, and the thiamine pyrophosphate effect.
January 1985, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition,
J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
[Thiamine deficiency in chronic alcoholism. The effect of thiamine treatment on the erythrocyte transketolase activity and the thiamine pyrophosphate effect].
June 1985, Ugeskrift for laeger,
J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
Thiamine pyrophosphate effect and normalized erythrocyte transketolase activity ratio in Wernicke-Korsakoff patients and acute alcoholics undergoing detoxification.
September 1996, Alcohol and alcoholism (Oxford, Oxfordshire),
J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
[Changes in transketolase activity during erythrocyte maturation].
January 1967, Voprosy meditsinskoi khimii,
J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
[Interaction of thiamine pyrophosphate and transketolase].
August 1972, Doklady Akademii nauk SSSR,
J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
The effect of thiamine defficiency on the activity of erythrocyte hemolysate transketolase.
July 1960, The Journal of nutrition,
J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
[On the relationship between thiamine uptake and the content of thiamine, thiamine pyrophosphate and the transketolase activity in rat organs].
January 1969, Enzymologia biologica et clinica,
J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
Erythrocyte transketolase in early thiamine deficiency.
April 1962, Annals of the New York Academy of Sciences,
J A Puxty, and A E Haskew, and J G Ratcliffe, and J McMurray
Charge transfer interactions in transketolase-thiamine pyrophosphate complex.
December 1970, Biochemical and biophysical research communications,
Copied contents to your clipboard!