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Changes in protein synthesis were examined during the cell cycle of Chinese hamster ovary cells by labeling synchronized cells at various times with [35S]methionine and separating the proteins on two-dimensional polyacrylamide gels. Several proteins, including tubulin, showed marked differences in their relative rates of synthesis during the cell cycle. A few proteins were found to be synthesized at a specific time during the cycle. In particular, a pair of proteins of approximately 21,000 daltons and isoelectric point of 5.5 were found to be synthesized only in late G2 and mitotic cells. Cells that were labeled during mitosis and then allowed to divide showed no trace of these proteins, indicating that their presence is transient and that they are likely involved in mitosis.
J T Westwood, and
E B Wagenaar, and
R B Church
January 1982,
Contributions to nephrology,
J T Westwood, and
E B Wagenaar, and
R B Church
August 1982,
Artificial organs,
J T Westwood, and
E B Wagenaar, and
R B Church
October 1975,
The Journal of biological chemistry,
J T Westwood, and
E B Wagenaar, and
R B Church
January 1982,
Transactions - American Society for Artificial Internal Organs,
J T Westwood, and
E B Wagenaar, and
R B Church
August 1987,
Plant physiology,
J T Westwood, and
E B Wagenaar, and
R B Church
January 1974,
Minerva nefrologica,
J T Westwood, and
E B Wagenaar, and
R B Church
November 1970,
Biochemical and biophysical research communications,
J T Westwood, and
E B Wagenaar, and
R B Church
July 1984,
Biochimica et biophysica acta,
J T Westwood, and
E B Wagenaar, and
R B Church
June 2017,
Cell reports,
J T Westwood, and
E B Wagenaar, and
R B Church
May 1980,
Gastroenterology,
