-transparent.png){kind=logo}
Forskolin stimulates adenylate cyclase in human thyroid membranes approximately 7-fold with half-maximal stimulation occurring at 5-10 microM. Guanine nucleotides are not required for stimulation of the enzyme by forskolin. Forskolin-stimulation is additive or greater than additive with that of TSH or Gpp(NH)p- (above 1 microM). Different from TSH- or Gpp(NH)p-stimulation of adenylate cyclase, uncoupling of the guanine nucleotide-binding regulatory component by increasing concentrations of MnCl2 did not result in uncoupling of forskolin stimulation. The finding indicates that forskolin may mainly act on the catalytic component of adenylate cyclase. From the present study, it is suggested that the diterpene forskolin stimulates adenylate cyclase in human thyroid membranes by a novel mechanism that differs from TSH- or Gpp(NH)p-stimulation, and that the diterpene may be a useful tool to investigate the metabolism of thyroid and its regulation in normal and pathological situations.
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
July 1982,
The Journal of biological chemistry,
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
August 1986,
Life sciences,
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
November 1985,
Archives of biochemistry and biophysics,
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
September 1982,
Endocrinology,
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
December 1982,
FEBS letters,
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
January 1983,
Molecular and cellular endocrinology,
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
June 1983,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
August 1978,
The Journal of biological chemistry,
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
July 1987,
Molecular pharmacology,
K Kasai, and
Y Suzuki, and
M Hiraiwa, and
H Kuroda, and
T Emoto, and
T Nakamura, and
S Shimoda
January 1981,
Endocrine research communications,
