The order of attachment of the purified platelet cytoskeletal proteins, F-actin, alpha-actinin and Actin Binding Protein (ABP) to the isolated platelet membrane has been investigated. Of the three proteins, only F-actin would directly interact with a membrane preparation that had been extensively washed to remove associated cytoskeletal proteins. alpha-Actinin would only add to the membrane to which F-actin had been re-attached and ABP only if both F-actin and alpha-actinin were present on the membrane. These studies provide some insight into the nature of the attachment of the platelet cytoskeleton to the cytoplasmic side of the membrane.