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The highly increased fibrinolytic activity of HeLa cells, treated with the tumor promoting phorbol ester, phorbol myristate acetate (PMA), correlates with equally increased levels of tissue-type plasminogen activator (t-PA) antigen in the conditioned media of these cells and concomitantly increased steady state levels of t-PA-specific mRNA. The effect of PMA on t-PA mRNA levels is completely blocked by pretreatment of the cells with the inhibitor of translation, cycloheximide, indicating that it requires the biosynthesis of at least one protein intermediate. In contrast, mRNA of the oncogene product c-myc can be induced for a brief period immediately following serum starvation in the presence and absence of PMA, and in the presence of cycloheximide. Our results suggest that increased t-PA biosynthesis in HeLa cells, probably through an increased rate of translation of the t-PA gene, forms part of the "late" events of the pleiotropic response to tumor promoters.
E K Waller, and
W D Schleuning
July 1977,
Cell,
E K Waller, and
W D Schleuning
March 1980,
Cancer research,
E K Waller, and
W D Schleuning
December 1995,
Cell structure and function,
E K Waller, and
W D Schleuning
March 1981,
Biochemical and biophysical research communications,
E K Waller, and
W D Schleuning
December 2012,
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis,
E K Waller, and
W D Schleuning
September 1982,
Clinical and experimental immunology,
E K Waller, and
W D Schleuning
April 1986,
Biochimica et biophysica acta,
E K Waller, and
W D Schleuning
May 1995,
The Journal of steroid biochemistry and molecular biology,
E K Waller, and
W D Schleuning
September 1981,
The Journal of cell biology,
E K Waller, and
W D Schleuning
January 1987,
Molecular endocrinology (Baltimore, Md.),
