Biomaterial Database

Humoral immune responses to soluble somatic extracts of Dipetalonema viteae adult worms infected hamsters. 1985

M H Chu, and J T Neilson

The present study examined the serum IgG1 and IgM antibody responses of infected hamsters to unfractionated and fractionated soluble somatic (SS) antigens of adult Dipetalonema viteae. Male and female adult worms were homogenized in a French pressure cell and the SS proteins extracted with 0.375 M Tris-glycine buffer. Serum IgG1 and IgM antibody responses in D. viteae infected with LVG, PD4 and CB hamster strains to the male and female unfractionated SS proteins were measured by an indirect enzyme linked immunosorbent assay (ELISA). Serum IgG1 antibody responses to the SS proteins were similar in all three strains of hamsters during the course of infection. There was no correlation between the level of IgG1 antibody and the onset of microfilariae clearance. The serum IgM antibody response was similar in both outbred LVG and inbred PD4 hamster strains. A lower IgM antibody response was found in CB hamsters and could be related to the failure of this hamster strain to eliminate microfilariae. The SS proteins of male and female adult worms were fractionated by preparative flat-bed isoelectric focusing on granulated gels (PIEF) to yield 8 and 7 fractions, respectively. The comparative antigenicity of the PIEF fractions from the SS proteins was measured by ELISA, using hyperimmune serum from LVG hamsters and rabbit antihamster IgG1-and IgM-alkaline phosphatase conjugates. No difference in ELISA reactivity was noted among the 8 and 7 PIEF fractions from male and female SS proteins.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007075	Immunoglobulin M	A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin.	Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D004153	Dipetalonema	A filarial nematode parasite of mammalian blood with the vector being a tick or small fly.	Dipetalonemas
D004154	Dipetalonema Infections	Infections with nematodes of the genus DIPETALONEMA.	Dipetalonemiasis,Infections, Dipetalonema,Dipetalonema Infection,Dipetalonemiases,Infection, Dipetalonema
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005368	Filariasis	Infections with nematodes of the superfamily FILARIOIDEA. The presence of living worms in the body is mainly asymptomatic but the death of adult worms leads to granulomatous inflammation and permanent fibrosis. Organisms of the genus Elaeophora infect wild elk and domestic sheep causing ischemic necrosis of the brain, blindness, and dermatosis of the face.	Elaeophoriasis,Filarioidea Infections,Infections, Filarioidea,Elaeophoriases,Filariases,Filarioidea Infection,Infection, Filarioidea
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).