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The presence of [Lys8-Asn9]-neurotensin8-13-like immunoreactivity was studied by radioimmunoassay in chicken and rat tissues. In the chicken, [Lys8-Asn9]-neurotensin8-13-like immunoreactivity showed a wide distribution throughout the central nervous system and the gastrointestinal tract, and the immunoreactive material co-eluted with the synthetic peptide on reverse-phase high performance liquid chromatography. In the rat, [Lys8-Asn9]-neurotensin8-13-like immunoreactivity was widely distributed when 0.1 M HCl was used as the extraction procedure. However, the immunoreactive material did not co-elute with the synthetic peptide on reverse-phase high performance liquid chromatography; moreover, the addition of the aspartic proteinase inhibitor pepstatin to the extraction medium resulted in a large reduction in the levels of [Lys8-Asn9]-neurotensin8-13-like immunoreactivity and no immunoreactive material could be detected when the tissues were extracted using acetone/HCl. The present results therefore indicate that [Lys8-Asn9]-neurotensin8-13-like immunoreactivity is not present in rat tissues. That which was detected resulted from an extraction artefact.
M Goedert, and
W N Schwartz, and
B J Williams
January 1983,
Peptides,
M Goedert, and
W N Schwartz, and
B J Williams
August 1984,
Brain research,
M Goedert, and
W N Schwartz, and
B J Williams
May 1988,
Regulatory peptides,
M Goedert, and
W N Schwartz, and
B J Williams
June 1986,
Endocrinology,
M Goedert, and
W N Schwartz, and
B J Williams
March 1987,
The Journal of comparative neurology,
M Goedert, and
W N Schwartz, and
B J Williams
February 1989,
Journal of neurocytology,
M Goedert, and
W N Schwartz, and
B J Williams
July 1980,
Life sciences,
M Goedert, and
W N Schwartz, and
B J Williams
June 1988,
Endocrinology,
M Goedert, and
W N Schwartz, and
B J Williams
January 1985,
Archives of oral biology,
M Goedert, and
W N Schwartz, and
B J Williams
July 1984,
Brain research,
