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Fibronectin from human plasma and its 180 kDa fragment which retained collagen-binding, cell-attachment and heparin-binding activities, were studied by velocity centrifugation and 1H-NMR methods. The fibronectin hydrodynamic radius strongly increased at pH 11 while the hydrodynamic properties of the fragment did not change noticeably. 1H-NMR spectroscopy also showed differences in the molecular properties of fibronectin and its 180 kDa fragment. Under physiological conditions the structure of fibronectin differs from that of its 180 kDa fragment. At pH 11 and in 4 M urea no differences in their structures are observed. It is suggested that interdomain and intersubunit interactions play an important role in maintaining the native conformation of intact fibronectin.
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
May 1991,
Archives of biochemistry and biophysics,
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
January 1984,
Proceedings of the National Academy of Sciences of the United States of America,
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
April 2002,
Indian journal of biochemistry & biophysics,
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
September 1985,
The Journal of biological chemistry,
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
December 2010,
Medecine sciences : M/S,
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
April 1996,
Journal of biochemistry,
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
March 1990,
European journal of biochemistry,
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
December 2014,
Molecules (Basel, Switzerland),
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
July 2006,
Matrix biology : journal of the International Society for Matrix Biology,
V N Bushuev, and
M L Metsis, and
A D Morozkin, and
E K Ruuge, and
N F Sepetov, and
V E Koteliansky
June 1996,
British journal of rheumatology,
