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Two subchronic studies were conducted to assess the potential toxicity of N-D-ornithyl amphotericin B methyl ester (OAME). In both studies the comparative control substance was amphotericin B (AMB). Dogs (5/sex/group) were given OAME (82% pure, based on high-pressure liquid chromatographic (HPLC) analysis) at 0.6, 2.5, and 10 mg/kg or AMB at 0.6 mg/kg intravenously once daily for 3 months. Two dogs per sex per group were retained for a 7-week postdose observation period. Rats (15/sex/group) were given daily doses of OAME at 4, 12, 24, and 36 mg/kg or AMB at 5 and 12 mg/kg intraperitoneally for 3 months. The principal organs of toxicity in both species were the liver, kidneys, and circulating erythrocytes. Hepatic changes in dogs consisted of periportal and centrilobular inflammation in animals of all dosed groups and were equivalent in dogs given 0.6 mg/kg OAME or AMB. In rats, acute hepatic necrosis with periportal, centrilobular, or panlobular distribution in animals of all OAME (except 4 mg/kg) and AMB-dosed groups was observed. These changes were equivalent in the 36-mg/kg OAME- and 12-mg/kg AMB-dosed animals. Renal changes, evidenced by increases in serum urea nitrogen water consumption, urine volume, decreased urine osmolality, and renal tubular changes (ranging from degeneration and regeneration to necrosis), were observed in both species. In dogs, these changes in the OAME-dosed animals were less severe at all doses than those observed in the AMB-dosed dogs. Renal changes in rats, which were mild in comparison to the dogs, were equivalent at doses of 5 and 12 mg/kg AMB and 36 mg/kg OAME. Decreased erythrocyte counts, hematocrit, and hemoglobin values were observed in both species. Unique to the dog study, however, were irreversible behavioral (somnolence, ataxia, tremors, and compulsive searching) and/or morphologic brain changes (gliosis with astrocytic hypertrophy and hyperplasia) at doses of 2.5 and 10 mg/kg OAME. Similar changes were observed in two dogs given 10 mg/kg OAME (100% pure, based on HPLC analysis) in a 6-week pilot study, indicating that the neurological changes were induced by OAME rather than by an impurity. These changes appear related to prolonged exposure to high plasma concentrations of OAME.
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
November 1987,
Antimicrobial agents and chemotherapy,
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
March 1985,
Antimicrobial agents and chemotherapy,
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
August 1997,
The Journal of antibiotics,
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
December 1985,
Antimicrobial agents and chemotherapy,
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
July 1984,
Antimicrobial agents and chemotherapy,
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
October 1976,
Antimicrobial agents and chemotherapy,
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
January 2002,
Acta biochimica Polonica,
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
February 1973,
Science (New York, N.Y.),
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
January 1988,
Toxicologic pathology,
T Massa, and
D P Sinha, and
J D Frantz, and
M E Filipek, and
R C Weglein, and
S A Steinberg, and
J T McGrath, and
B F Murphy, and
R J Szot, and
H E Black
February 1975,
The Journal of antibiotics,
