-transparent.png){kind=logo}
Thromboxane B2 (TxB2) was biosynthesized from prostaglandin endoperoxides (PGG2, PGH2) using guinea pig lung microsomes and infused into an unanesthetized monkey. Urine was collected and TxB2 metabolites were isolated by reversed phase partition chromatography and high performance liquid chromatography. A major metabolite (TxB2-M) was found to be excreted in greater than two-fold abundance relative to other metabolites. Its structure was determined by gas chromatography-mass spectrometry to be dinor-thromboxane B2. In vitro incubation of TxB2 with rat liver mitochondria yielded a C18 derivative with a mass spectrum identical to that of TxB2-M, substantiating that the major urinary metabolite of TxB2 in the monkey is a product of a single step of beta-oxidation.
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
June 1994,
Prostaglandins,
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
March 1979,
Prostaglandins,
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
January 1982,
Methods in enzymology,
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
July 1989,
Biochimica et biophysica acta,
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
April 1977,
Prostaglandins,
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
June 1982,
Archives des maladies du coeur et des vaisseaux,
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
March 1986,
Prostaglandins,
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
November 1981,
Lancet (London, England),
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
July 1994,
Clinical physiology (Oxford, England),
L J Roberts, and
B J Sweetman, and
J L Morgan, and
N A Payne, and
J A Oates
January 1990,
Eicosanoids,
