Biomaterial Database

Antibodies against Pseudomonas aeruginosa in serum from normal persons and patients colonized with mucoid or non-mucoid Pseudomonas aeruginosa: Results obtained by crossed immunoelectrophoresis. 1977

N Holby

Serum from 154 normal persons was investigated for precipitating antibodies against P. aeruginosa by crossed immunoelectrophoresis, using a polyvalent P. aeruginosa standard antigen. Ninety-four per cent of the sera contained no demonstrable precipitins, and 6% contained precipitins against only one P. aeruginosa antigen. Thirty-four per cent of sera from 122 patients colonized with P. aeruginosa in various anatomical regions contained precipitins against 2 to 41 P. aeruginosa antigens. Serum from 14 patients harbouring mucoid strains of P. aeruginsa contained precipitants against these bacteria, and the average number of precipitins was significantly higher than the average number of precipitins in serum from patients harbouring non-mucoid strains. The largest number of precipitins was found in serum from adult patients chronically colonized with mucoid P. aeruginosa in the lower respiratory tract, and from adult patients with P. aeruginosa bacteremia originating from suppurative urinary tract infections caused by mucoid strains. Serum from patients with P. aeruginosa cystitis or from patients with P. aeruginosa colonization of the middle ear or with wound infection, contained only few or no precipitins.

UI	MeSH Term	Description	Entries
D007123	Immunoelectrophoresis, Two-Dimensional	Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.	Immunoelectrophoresis, Crossed,Immunoelectrophoresis, 2-D,Immunoelectrophoresis, 2D,2-D Immunoelectrophoresis,2D Immunoelectrophoresis,Crossed Immunoelectrophoresis,Immunoelectrophoresis, 2 D,Immunoelectrophoresis, Two Dimensional,Two-Dimensional Immunoelectrophoresis
D008297	Male		Males
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D011550	Pseudomonas aeruginosa	A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.	Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D001769	Blood	The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D002648	Child	A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL.	Children
D002675	Child, Preschool	A child between the ages of 2 and 5.	Children, Preschool,Preschool Child,Preschool Children
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man