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Experimental allergic orchitis (EAO) was induced in cyclophosphamide (CY)-pretreated C3H/He mice by immunization with viable testicular cells (TC) without any adjuvants. The pathological changes such as hypospermatogenesis in the seminiferous tubules and mild cellular infiltration in the interstitium were observed in most mice thus treated. Delayed footpad reaction (DFR) against testicular antigen and anti-sperm antibody in sera were detected in those mice. This murine model of orchitis is new and simple in that no adjuvants are used for induction and can be useful in further studies on mechanisms of induction and maintenance of EAO.
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