BIOMAT Database Logo BIOMAT Database Logo

Isolation and characterization of glycosaminoglycans from Schistosoma mansoni. 1985

N P Robertson, and G D Cain

Tegumental tissues of paired adult Schistosoma mansoni were removed by treatment with Triton X-100 and recovered by centrifugation. The chloroform-methanol insoluble residues of this isolated tegumental fraction and of the denuded carcasses were analysed for glycosaminoglycan (GAG) and sialic acid contents. Treatment with GAG-specific enzymes followed by electrophoretic analysis showed that both the carcass and tegument contained heparin and/or heparan sulfate, chondroitin sulfate and hyaluronic acid. All these except hyaluronic acid were present in the tegumental fraction. Based on uronic acid content, about 73% of the total GAG was in the tegumental membrane, 15% in the tegmental matrix and the remaining 12% was in the carcass. The presence of heparin-like polysaccharide may present entrapment of the schistosoma by the hosts' blood-clotting process.

UI MeSH Term Description Entries
D008297 Male Males
D008647 Mesocricetus A genus in the order Rodentia and family Cricetidae. One species, Mesocricetus auratus or golden hamster is widely used in biomedical research. Hamsters, Golden,Hamsters, Golden Syrian,Hamsters, Syrian,Mesocricetus auratus,Syrian Golden Hamster,Syrian Hamster,Golden Hamster,Golden Hamster, Syrian,Golden Hamsters,Golden Syrian Hamsters,Hamster, Golden,Hamster, Syrian,Hamster, Syrian Golden,Syrian Hamsters
D011133 Polysaccharide-Lyases A group of carbon-oxygen lyases. These enzymes catalyze the breakage of a carbon-oxygen bond in polysaccharides leading to an unsaturated product and the elimination of an alcohol. EC 4.2.2. Polysaccharide Lyase,Polysaccharide-Lyase,Lyase, Polysaccharide,Polysaccharide Lyases
D002810 Chondroitinases and Chondroitin Lyases Enzymes which catalyze the elimination of glucuronate residues from chondroitin A, B, and C or which catalyze the hydrolysis of sulfate groups of the 2-acetamido-2-deoxy-D-galactose 6-sulfate units of chondroitin sulfate. EC 4.2.2.-. Chondroitinase,Chondroitin-4-Sulfate Depolymerase,Chondroitinases,Chondroitin 4 Sulfate Depolymerase,Depolymerase, Chondroitin-4-Sulfate
D006025 Glycosaminoglycans Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine (see ACETYLGLUCOSAMINE) or N-acetylgalactosamine (see ACETYLGALACTOSAMINE). Glycosaminoglycan,Mucopolysaccharides
D006224 Cricetinae A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS. Cricetus,Hamsters,Hamster
D006821 Hyaluronoglucosaminidase An enzyme that catalyzes the random hydrolysis of 1,4-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. (From Enzyme Nomenclature, 1992) There has been use as ANTINEOPLASTIC AGENTS to limit NEOPLASM METASTASIS. Hyaluronidase,Duran-Reynals Permeability Factor,GL Enzyme,Hyaglosidase,Hyaluronate Hydrolase,Wydase,Duran Reynals Permeability Factor,Factor, Duran-Reynals Permeability,Hydrolase, Hyaluronate,Permeability Factor, Duran-Reynals
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012550 Schistosoma mansoni A species of trematode blood flukes of the family Schistosomatidae. It is common in the Nile delta. The intermediate host is the planorbid snail. This parasite causes schistosomiasis mansoni and intestinal bilharziasis. Schistosoma mansonus,mansonus, Schistosoma
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species

Related Publications

N P Robertson, and G D Cain
Isolation and characterization of surface antigens from Schistosoma mansoni schistosomula.
August 1981, Molecular and biochemical parasitology,
N P Robertson, and G D Cain
Isolation and characterization of Schistosoma mansoni constitutive androstane receptor.
July 2006, Molecular and biochemical parasitology,
N P Robertson, and G D Cain
Identification, isolation and characterization of a Fyn-like tyrosine kinase from Schistosoma mansoni.
March 2001, Parasitology,
N P Robertson, and G D Cain
Acetylcholinesterase from Schistosoma mansoni: immunological characterization.
May 1991, Immunology letters,
N P Robertson, and G D Cain
Isolation and polymorphism in mitochondrial DNA from Schistosoma mansoni.
July 1991, Molecular and biochemical parasitology,
N P Robertson, and G D Cain
Isolation and functional characterization of snail hemocyte-modulating polypeptide from primary sporocysts of Schistosoma mansoni.
November 1991, Molecular and biochemical parasitology,
N P Robertson, and G D Cain
Isolation and characterization of a serologically active exoantigen of Schistosoma mansoni cercariae.
April 1967, Experimental parasitology,
N P Robertson, and G D Cain
Isolation of a polysaccharide antigen from Schistosoma mansoni eggs.
January 1979, Journal of immunology (Baltimore, Md. : 1950),
N P Robertson, and G D Cain
Schistosoma mansoni: isolation and characterization of Smpi56, a novel serine protease inhibitor.
March 1994, Experimental parasitology,
N P Robertson, and G D Cain
Isolation and characterization of surface antigens from Schistosoma mansoni. II. Antigenicity of radiolabeled proteins from adult worms.
August 1979, The Journal of parasitology,
Copied contents to your clipboard!