Biomaterial Database

Murine neutrophils collected from subcutaneously implanted sponges. 1985

J F Bale, and M E O'Neil

Polyvinyl sponges implanted subcutaneously in mice were used as a source of murine neutrophils (PMNs). Yield of PMNs from sponges averaged 6.3 X 10(5) PMNs per mouse and viability of sponge PMNs was 96.5%. These results were comparable to the yield and viability of PMNs harvested from peripheral blood. The chemotactic activity of sponge PMNs was significantly greater than the chemotactic activity of peripheral blood PMNs (chemotactic index of 4.78 +/- 1.66 in sponge PMNs versus 2.16 +/- 1.08 in peripheral blood PMNs, P less than 0.001). The difference in chemotactic activity was not attributable to hypotonic injury of PMNs nor the presence of soluble factors in sponge fluids. Phagocytic activity of sponge PMNs was comparable to the activity of blood PMNs (mean of 31.3 +/- 16.4% phagocytic cells for sponge PMNs versus 33.0 +/- 23.9% for blood PMNs). In addition, the number of fluorescent spheres ingested by sponge PMNs was not different (mean of 2.81 +/- 0.86 in sponge PMNs versus 2.65 +/- 0.68 spheres per cell for blood PMNs). These studies indicate that subcutaneously implanted sponges can be used as a source of functioning murine PMNs.

UI	MeSH Term	Description	Entries
D009504	Neutrophils	Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.	LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D011145	Polyvinyls	POLYMERS derived from the monomer VINYL COMPOUNDS.	Polyvinyl
D001800	Blood Specimen Collection	The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc.	Blood Specimen Collections,Collection, Blood Specimen,Collections, Blood Specimen,Specimen Collection, Blood,Specimen Collections, Blood
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002470	Cell Survival	The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.	Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002634	Chemotaxis, Leukocyte	The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction.	Leukotaxis,Leukocyte Chemotaxis
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013523	Surgical Equipment	Nonexpendable apparatus used during surgical procedures. They are differentiated from SURGICAL INSTRUMENTS, usually hand-held and used in the immediate operative field.	Equipment, Surgical,Equipments, Surgical,Surgical Equipments