-transparent.png){kind=logo}
We tested the inhibitory effects of phenothiazine derivatives on bone in vivo and osteoblastic cells in vitro. Chlorpromazine (CPZ) and trifluoperazine (TFPZ) dose-dependently decreased alkaline phosphatase activity in calvariae of rats: half-maximal inhibitory effects of CPZ and TFPZ were at 2.0 and 4.0 mg/kg, respectively. These effects were more specific for calvaria and ileum than for liver and duodenum. CPZ inhibited the proliferation of osteoblastic clone MC3T3-E1 cells to a greater extent than that of liver epithelial clone RLC-18(4) cells in vitro. CPZ, TFPZ and perphenazine (PNZ) also affected rather specifically alkaline phosphatase activity and collagen synthesis and were not cytotoxic. These in vivo and in vitro findings suggest inhibitory effects on osteoblastic cell function(s). However, promethazine (PMZ) had little effect in vivo and in vitro. In addition, increases in serum calcium and phosphate induced by CPZ indicate its possible involvement in bone resorption.
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
March 1972,
Archives internationales de pharmacodynamie et de therapie,
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
January 1988,
Revista de igiena, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia,
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
February 1962,
Biochemical pharmacology,
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
June 1997,
Archives of oral biology,
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
February 2009,
Photomedicine and laser surgery,
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
June 1966,
Endocrinology,
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
November 1986,
Biochemical and biophysical research communications,
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
February 2021,
Biochemical Society transactions,
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
February 2008,
Cancer biology & therapy,
T Komoda, and
E Ikeda, and
Y Nakatani, and
Y Sakagishi, and
N Maeda, and
T Kato, and
M Kumegawa
March 2005,
Biomaterials,
