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Twenty-one identical tumour specimens were cultured both in the Plasma-Clot Diffusion Chamber (PCDC) Technique and the Human Tumour Colony-forming Assay (HTCA). The culture results achieved in the PCDC-technique were clearly superior to the HTCA: in the PCDC the mean and median plating efficiency (PE) was 0.156 and 0.147, in the HTCA 0.103 and 0.028%; adequate growth rate in the PCDC-technique was 67% and in the HTCA 38%. Fewer cells were required for plating in the PCDC-technique: 6.4 X 10(4) vs. 2.6 X 10(5) in the HTCA. The mean and median coefficient of variation of the colony numbers in the PCDC-technique appeared much higher: 27.3 and 37.3 vs. 11.2 and 11.1% in the HTCA. The relation between the PEs obtained for the same specimen in the two techniques was compared. No positive correlation was found, which can possibly be ascribed to technical shortcomings in both techniques.
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
May 1996,
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology,
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
June 1989,
British journal of cancer,
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
September 1985,
British journal of cancer,
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
January 1986,
The Netherlands journal of medicine,
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
January 2018,
Methods in molecular biology (Clifton, N.J.),
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
December 1986,
British journal of cancer,
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
November 1976,
British journal of cancer,
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
November 1988,
Hinyokika kiyo. Acta urologica Japonica,
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
September 1987,
International journal of cell cloning,
P H Slee, and
R Willemze, and
A T van Oosterom, and
E Lurvink, and
L van den Berg
March 1976,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
