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Red cell populations were fractionated on the basis of differences in density by a centrifugation method described by Murphy and on the basis of differences in mean cell volume (MCV) by counterflow centrifugation. By 59Fe-incorporation and determination of the HbA1c content, both methods were studied for their ability to separate red cell populations into fractions of different mean cell age. It can be concluded that separation on the basis of differences in cell volume results in a linear separation according to age whereas separation on the basis of density only results in an accumulation of very young cells in the top fractions. A gradual decrease in cell volume with age, combined with a constant haemoglobin concentration in the cells, indicates release of haemoglobin from the red cells during their lifespan.
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
January 1992,
Blood,
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
July 2000,
Clinical chemistry and laboratory medicine,
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
September 1986,
Scandinavian journal of haematology,
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
April 1976,
Analytical biochemistry,
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
January 1977,
Methods in cell biology,
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
October 1983,
Journal of immunological methods,
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
September 1986,
Journal of immunological methods,
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
June 1988,
Experimental hematology,
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
October 1984,
British journal of haematology,
S G van der Vegt, and
A M Ruben, and
J M Werre, and
D M Palsma, and
C W Verhoef, and
J de Gier, and
G E Staal
March 1987,
Transplantation,
