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A procedure for using the Omnicon automated image analysis system for counting colonies grown from a human tumour cell line (COLO 205) in the Courtenay-Mills assay is described. This involves the transfer of the agar medium from culture tubes into petri dishes. Comparisons of observer and instrument counts were done on a blinded basis. Run-to-run correlation coefficient was 0.996 for automated counting and the inter-observer correlation coefficient was 0.984. Both assessments showed a linear relationship between the number of cells plated and the number of colonies grown. Automated colony counting is fast, reliable and provides additional information on colony size distribution, not obtainable with manual counting. This automated procedure will greatly facilitate in vitro drug sensitivity evaluation.
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
November 1991,
International journal of cell cloning,
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
May 1984,
International journal of cell cloning,
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
November 1970,
Applied microbiology,
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
August 2023,
Microbiology spectrum,
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
January 2001,
Physics in medicine and biology,
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
September 2001,
Computer methods and programs in biomedicine,
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
January 2015,
Journal of microbiological methods,
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
August 2010,
Cytometry. Part A : the journal of the International Society for Analytical Cytology,
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
January 1989,
Anticancer research,
R H Verheijen, and
R D Whelan, and
W F Feitz, and
P Kenemans, and
G P Vooys, and
C J Herman, and
B T Hill
October 2006,
Journal of immunological methods,
