Biomaterial Database

An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry. 1985

C R Fricker, and E Quail, and L McGibbon, and R W Girdwood

A total of 745 samples of chicken giblets was cultured to determine the relative efficiency of a commercially available Rappaport-Vassiliadis medium (RV-Oxoid). Experiments to determine the optimum inoculation ratio showed that 1:100 was superior to the other ratios tested. Comparison of RV-Oxoid with standard RV and RV-medium prepared using soya peptone (RV-soya) showed that after 24 h RV-soya was significantly better than RV-Oxoid (P less than 0.05), although there was no significant difference between standard RV and RV-Oxoid. Furthermore, when the duration of incubation was extended to 48 h there was no significant difference between the three media (P greater than 0.25). We conclude that RV-Oxoid is a satisfactory product for the isolation of salmonellae from poultry, providing that it is inoculated at a ratio of 1:100 and is incubated for 48 h. Its use can therefore be recommended to laboratories who wish to use a dehydrated medium.

UI	MeSH Term	Description	Entries
D008460	Meat	The edible portions of any animal used for food including cattle, swine, goats/sheep, poultry, fish, shellfish, and game.	Meats
D011200	Poultry	Domesticated birds raised for food. It typically includes CHICKENS; TURKEYS, DUCKS; GEESE; and others.	Fowls, Domestic,Domestic Fowl,Domestic Fowls,Fowl, Domestic,Poultries
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012475	Salmonella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.

Related Publications

C R Fricker, and E Quail, and L McGibbon, and R W Girdwood

Improved isolation of salmonellae from faeces using a semisolid Rappaport-Vassiliadis medium.

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C R Fricker, and E Quail, and L McGibbon, and R W Girdwood

A comparison of the original Rappaport medium (R medium) and the Rappaport-Vassiliadis medium (RV medium) in the isolation of salmonellae from meat products.

August 1984, The Journal of hygiene,

C R Fricker, and E Quail, and L McGibbon, and R W Girdwood

Comparison of Rappaport-Vassiliadis Enrichment Medium and Tetrathionate Brilliant Green Broth for Isolation of Salmonellae from Meat Products.

July 1983, Journal of food protection,

C R Fricker, and E Quail, and L McGibbon, and R W Girdwood

The Rappaport-Vassiliadis (RV) enrichment medium for the isolation of salmonellas: an overview.

February 1983, The Journal of applied bacteriology,

C R Fricker, and E Quail, and L McGibbon, and R W Girdwood

Efficiency of Rappaport-Vassiliadis medium.

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