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An assay of adenosine deaminase activity in pleural effusions is described. For the continuous determination of adenosine deaminase, the liberated ammonia is estimated by coupling the liberated NH3 with 2-oxoglutarate. The reaction is followed by the decrease of NADH absorbance at 340 nm. The assay was optimized for a Hitachi 705 analyser, with respect to pH, adenosine concentration and glutamate dehydrogenase activity. The assay is linear to an adenosine deaminase catalytic concentration of 110 U/l. Elevated adenosine deaminase activities are found in pleural effusions of patients with tuberculosis, empyema and mesothelioma. Although elevated adenosine deaminase activity in pleural effusion is not pathognomonic for tuberculosis, it may be valuable as a first screening parameter.
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
January 1984,
The Journal of automatic chemistry,
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
March 1992,
The Journal of the Association of Physicians of India,
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
June 1995,
Chest,
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
November 1996,
Clinical chemistry,
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
October 2014,
Lung India : official organ of Indian Chest Society,
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
June 1986,
The Journal of the Association of Physicians of India,
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
January 1991,
Advances in experimental medicine and biology,
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
January 1984,
Acta medica Scandinavica,
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
April 1985,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
E H Slaats, and
E G Asberg, and
A R van Keimpema, and
H Kruijswijk
November 1986,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
