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A Pseudomonas aeruginosa strain isolated at Besançon Hospital, France, proved to be highly resistant to carbenicillin and showed a high hydrolytic activity toward this antibiotic. We clearly demonstrated that two beta-lactamases were synthetized: one of them, constitutive, has its enzymatic activity directed mainly toward penicillins, and carbenicillin appears to be its best substrate (higher V(max)); thus, this beta-lactamase is a "carbenicillinase" that differs from the well-known "TEM-like" enzymes. The isoelectric point of this carbenicillinase is 5.30 +/- 0.03. The other one is an inducible cephalosporinase, very similar to the cephalosporinases usually found in these organisms. Its isoelectric point is 8.66 +/- 0.04. These two enzymes have been separated by affinity chromatography and isoelectric focusing. The kinetic constants were measured by computerized microacidimetry.
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
November 2005,
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases,
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
January 1975,
Annales de microbiologie,
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
October 1978,
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
October 1986,
The Journal of antimicrobial chemotherapy,
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
July 1972,
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
August 1971,
Lancet (London, England),
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
January 1982,
Annales de microbiologie,
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
January 1983,
Bollettino dell'Istituto sieroterapico milanese,
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
September 2009,
Antimicrobial agents and chemotherapy,
R Labia, and
M Guionie, and
J M Masson, and
A Philippon, and
M Barthelemy
August 1969,
Lancet (London, England),
