Biomaterial Database

An evaluation of alkaline peptone water for enrichment of Vibrio cholerae in feces. 1985

M Lesmana, and R C Rockhill, and D Sutanti, and A Sutomo

A comparison was made to determine the sensitivity of direct inoculation of thiosulfate citrate bile salts agar (TCBS) and alkaline peptone water (APW) enrichment prior to direct inoculation of TCBS to culture Vibrio cholerae from feces of patients with gastroenteritis. V. cholerae was isolated from 611 feces specimens. Of those, 535 were isolated in TCBS and APW-TCBS, 15 in only TCBS and 61 in only APW-TCBS. V. parahemolyticus (21) and non-agglutinating vibrios (11) were also isolated but more often in direct inoculated TCBS than APW-TCBS cultures. Maximum isolation sensitivity of V. cholerae and V. parahemolyticus from feces is obtained by both direct inoculation of TCBS and enrichment in APW prior to TCBS inoculation.

UI	MeSH Term	Description	Entries
D010461	Peptones	Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)	Peptone
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D014734	Vibrio cholerae	The etiologic agent of CHOLERA.	Bacillus cholerae,Bacillus cholerae-asiaticae,Liquidivibrio cholerae,Microspira comma,Pacinia cholerae-asiaticae,Spirillum cholerae,Spirillum cholerae-asiaticae,Vibrio albensis,Vibrio cholera,Vibrio cholerae-asiaticae,Vibrio comma