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Ascorbate oxidase from zucchini squash was immobilized onto CH-Sepharose via carbodiimide. The properties of the immobilized enzyme were found to be similar to those of the free ascorbate oxidase. The immobilized enzyme was utilized in a flow-through system equipped with a polarographic detector which monitors the oxygen depletion due to the reaction ascorbic acid + 1/2 O2----dehydroascorbic acid + H2O. This method, the response of which is linear between 3 X 10(-7) and 5 X 10(-4) M ascorbate, was utilized to measure the ascorbic acid in biological samples such as human plasma and fruit juices at a rate of about 60 determinations every hour with a standard deviation lower than 5%.
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
November 1979,
FEBS letters,
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
January 1992,
Applied biochemistry and biotechnology,
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
August 1999,
Talanta,
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
March 2016,
Food technology and biotechnology,
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
May 1991,
Plant physiology,
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
January 1972,
The Italian journal of biochemistry,
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
January 1998,
Glycoconjugate journal,
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
June 1974,
FEBS letters,
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
October 1990,
The Analyst,
R Stevanato, and
L Avigliano, and
A Finazzi-Agrò, and
A Rigo
January 2011,
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
