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Chemical reprogramming of human blood cells to pluripotent stem cells. 2025
Fangqi Peng, and
Yanglu Wang, and
Lin Cheng, and
Ruyi Cai, and
Xiaodi Fu, and
Zhihan Yang, and
Ruoqi Cheng, and
Weizhen Zeng, and
Yingshuai Dong, and
Jingxiao Cao, and
Jingping Mao, and
Jingran Zeng, and
Tianxing Liu, and
Guanxian Chen, and
Qi Lei, and
Lipeng Wang, and
Lulu Liu, and
Shicheng Sun, and
Cheng Li, and
Rong Mu, and
Jingyang Guan, and
Hongkui Deng
MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences and MOE Engineering Research Center of Regenerative Medicine, School of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
Chemical reprogramming offers a fundamentally innovative approach for generating human chemically induced pluripotent stem (hCiPS) cells using small molecules. Our recent studies showed that this approach was highly efficient in reprogramming human fibroblasts to hCiPS cells. However, generating hCiPS cells from human blood cells, which are the most accessible and convenient source for reprogramming, remains a challenge. In this study, we established a robust method that successfully generated hCiPS cells from both cord blood and adult peripheral blood cells. This method achieved efficient reprogramming with both fresh and cryopreserved blood cells across different donors. Notably, this method also efficiently generated an average of over 100 hCiPS colonies from just a single drop of fingerstick blood. These results highlight the advantages of chemical reprogramming for generating hCiPS cells from a blood source and represent a next-generation platform for efficient, scalable, and convenient stem cell production with broad applications in regenerative medicine.
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