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Endogenous levels of salsolinol and dopamine were measured by a gas chromatography/mass spectrometry (GC/MS)--selected ion monitoring technique using deuterated internal standards in rats allowed to self-inject either acetaldehyde, ethanol or saline control solution over 20 days for 1 h/day. Significant increases in medial basal hypothalamic (MBH) and striatal salsolinol concentrations were found in animals exposed to acetaldehyde but not ethanol, whereas dopamine concentrations for these animals did not differ significantly from rats exposed to control conditions. The data provides further support for the in vivo formation of salsolinol following acetaldehyde exposure in experimental animals.
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