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A gene-specific transcription factor, called USF, has been partially purified from HeLa cell nuclear extracts. Addition of USF results in a 10 to 20 fold increase in transcription from the adenovirus major late promoter in an in vitro system reconstituted with transcription factors TFIIB, TFIID, TFIIE, and RNA polymerase II. Binding of USF to the promoter inhibits DNAase I cleavages over a 20 base pair region just upstream of the -45 to +35 region shown previously to interact with TFIID. More discriminating footprint analyses using methidiumpropyl-EDTA-Fe(II) as the cleaving agent indicate that USF interacts primarily with the small palindromic DNA sequence GGCCACGTGACC located between positions -63 and -52 of the major late promoter, while TFIID interacts primarily with a 10 base pair DNA region centered on the consensus TATA sequence. Dissociation rate measurements indicate a cooperative interaction between USF and TFIID when simultaneously bound to the promoter DNA.
M Sawadogo, and
R G Roeder
December 1985,
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M Sawadogo, and
R G Roeder
December 1986,
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M Sawadogo, and
R G Roeder
October 1984,
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M Sawadogo, and
R G Roeder
December 1984,
Nucleic acids research,
M Sawadogo, and
R G Roeder
October 1986,
The EMBO journal,
M Sawadogo, and
R G Roeder
June 1991,
Nucleic acids research,
M Sawadogo, and
R G Roeder
December 1991,
Nucleic acids research,
M Sawadogo, and
R G Roeder
August 1993,
The Journal of biological chemistry,
M Sawadogo, and
R G Roeder
October 1989,
Proceedings of the National Academy of Sciences of the United States of America,
M Sawadogo, and
R G Roeder
December 1985,
Cell,
