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A vitamin D-dependent calcium-binding protein of 28,000 Mr was purified by a convenient method from the high-ammonium sulfate-saturated fraction (70 to 100% saturation) of bovine cerebellum and kidney. This method is based on the calcium-dependent DEAE-cellulose column chromatography that reflected the specific feature of this protein: different eluting salt concentrations in the presence or absence of Ca2+. The procedure of purification was easily performed by the detection of calcium-binding protein using 45Ca autoradiography. The purified calcium-binding protein showed the same molecular weight and calcium-dependent change of mobility in nondenaturing gel electrophoresis as vitamin D-dependent calcium-binding protein. The 28,000-Mr calcium-binding protein was induced in kidney by the injection of vitamin D in vitamin D-deficient rats.
K Maruyama, and
K Ebisawa, and
Y Nonomura
August 1988,
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K Maruyama, and
K Ebisawa, and
Y Nonomura
May 1986,
FEBS letters,
K Maruyama, and
K Ebisawa, and
Y Nonomura
August 1985,
Neuroscience letters,
K Maruyama, and
K Ebisawa, and
Y Nonomura
November 1987,
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K Maruyama, and
K Ebisawa, and
Y Nonomura
July 1976,
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K Maruyama, and
K Ebisawa, and
Y Nonomura
February 1975,
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K Maruyama, and
K Ebisawa, and
Y Nonomura
April 1961,
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K Maruyama, and
K Ebisawa, and
Y Nonomura
January 1983,
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K Maruyama, and
K Ebisawa, and
Y Nonomura
November 1981,
Nature,
K Maruyama, and
K Ebisawa, and
Y Nonomura
November 1988,
DNA (Mary Ann Liebert, Inc.),
