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An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of total anti-Campylobacter immunoglobulins in human sera. In this assay disintegrated Campylobacter bacteria were used as the antigen. Absorption tests including other possibly enteropathogenic bacterial species showed that the ELISA system displayed a high immunological specificity for Campylobacter. Using this ELISA it was found that in about 80% of Campylobacter patients these Campylobacter antibodies are produced to almost maximal levels within 8 days after onset of disease, and that they may persist for at least 4 months. Indeed, Campylobacter antibodies were demonstrated at low levels in a large number of control sera. However, accepting an antibody titre of 1:640 as indicative of Campylobacter infection, the statistical sensitivity of the ELISA system was 77% and the specificity 95%. In an epidemiological survey a high association was demonstrated between the severity of Campylobacter-related symptoms and antibody titre values. Assessment of Campylobacter antibody titres by means of this ELISA and by a complement fixation test in 92 sera from index patients and contacts with and without symptoms showed a high association of results.
J Oosterom, and
C H den Uyl, and
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J Huisman, and
A E Busschbach, and
F G Poelma, and
R Bellemans
February 1983,
Journal of clinical pathology,
J Oosterom, and
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S Lauwers, and
J Huisman, and
A E Busschbach, and
F G Poelma, and
R Bellemans
July 1982,
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
J Oosterom, and
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A E Busschbach, and
F G Poelma, and
R Bellemans
July 1985,
New Zealand veterinary journal,
J Oosterom, and
C H den Uyl, and
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S Lauwers, and
J Huisman, and
A E Busschbach, and
F G Poelma, and
R Bellemans
December 1982,
Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology,
J Oosterom, and
C H den Uyl, and
J R Bänffer, and
S Lauwers, and
J Huisman, and
A E Busschbach, and
F G Poelma, and
R Bellemans
February 1979,
Journal of clinical pathology,
J Oosterom, and
C H den Uyl, and
J R Bänffer, and
S Lauwers, and
J Huisman, and
A E Busschbach, and
F G Poelma, and
R Bellemans
March 1986,
Veterinary parasitology,
J Oosterom, and
C H den Uyl, and
J R Bänffer, and
S Lauwers, and
J Huisman, and
A E Busschbach, and
F G Poelma, and
R Bellemans
June 1984,
American journal of veterinary research,
J Oosterom, and
C H den Uyl, and
J R Bänffer, and
S Lauwers, and
J Huisman, and
A E Busschbach, and
F G Poelma, and
R Bellemans
November 1983,
The Veterinary record,
J Oosterom, and
C H den Uyl, and
J R Bänffer, and
S Lauwers, and
J Huisman, and
A E Busschbach, and
F G Poelma, and
R Bellemans
October 1984,
Journal of immunological methods,
J Oosterom, and
C H den Uyl, and
J R Bänffer, and
S Lauwers, and
J Huisman, and
A E Busschbach, and
F G Poelma, and
R Bellemans
January 1994,
Veterinary research communications,
