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A protein which specifically binds [3H]benzo[a]pyrene and other polycyclic aromatic hydrocarbons has been purified over 6000-fold from rat hepatic cytosol by using ion-exchange, gel permeation, and hydrophobic interaction chromatography. The binding protein differs from the 9S binding protein characterized in other laboratories. A Stokes radius of 2.75 nm was determined by gel filtration on Sephadex G-100. A sedimentation coefficient of 3.3 S was determined by using sucrose gradient analysis. The ability of this protein to bind total rat liver DNA as well as subclones containing portions of the rat cytochrome P-450c gene was investigated. Under high stringency conditions, this binding protein was found to interact in a specific and saturable manner with several subclones of the rat cytochrome P-450c gene containing 5'-upstream sequences, as well as portions of intron 1. Binding was not observed to the coding portions of the gene. These data implicate the "4S" binding protein in the transregulation of rat cytochrome P-450c expression.
W H Houser, and
R N Hines, and
E Bresnick
October 1990,
Carcinogenesis,
W H Houser, and
R N Hines, and
E Bresnick
July 1990,
Carcinogenesis,
W H Houser, and
R N Hines, and
E Bresnick
November 1986,
Archives of biochemistry and biophysics,
W H Houser, and
R N Hines, and
E Bresnick
April 1986,
Archives of biochemistry and biophysics,
W H Houser, and
R N Hines, and
E Bresnick
April 1988,
Cancer metastasis reviews,
W H Houser, and
R N Hines, and
E Bresnick
July 1999,
Toxicology and applied pharmacology,
W H Houser, and
R N Hines, and
E Bresnick
February 1990,
Journal of medicinal chemistry,
W H Houser, and
R N Hines, and
E Bresnick
January 1981,
Advances in experimental medicine and biology,
W H Houser, and
R N Hines, and
E Bresnick
February 1997,
Toxicology letters,
W H Houser, and
R N Hines, and
E Bresnick
August 1988,
The Journal of pharmacology and experimental therapeutics,
