Biomaterial Database

[Studies on 16 alpha-hydroxyandrost-4-ene-3, 17-dione in human pregnancy measured by high performance liquid chromatography and radioimmunoassay]. 1985

A Ikegawa

16 alpha-hydroxyandrost-4-ene-3,17-dione (16 alpha OH-A) was measured in pregnant women by radioimmunoassay (RIA) with specific antiserum. After extraction of the serum the 16 alpha OH-A fraction was separated by the use of Sephadex LH-20 mini column. Lately, high performance liquid chromatograph (HPLC) was combined with RIA (HPLC-RIA) for the measurement of various kinds of steroids from an extract of a serum or urine sample. The purpose of this study was to establish the HPLC-RIA method for the measurement of 16 alpha OH-A in pregnant women. Pooled serum was extracted twice with 5 volumes of ethylether, and the combined extract was separated either by LH-20 mini column with the solvent system n-hexane:benzene:methanol = 8:1:1 or by HPLC ODS column with MeOH:CH3CN:H2O = 60:10:30. The 16 alpha OH-A fraction was collected and an aliquot was measured by RIA. The coefficients of variance for intra and inter-assay (n = 10) were computed to be 8.6 and 12.1% with LH-20, and 7.2 and 11.7% with HPLC, respectively. Recovery rate was 95.7 +/- 7.4% with LH-20 and 92.5 +/- 8.7% with HPLC. The values of 16 alpha OH-A as measured following two kinds of chromatographies were closely related to each other. The mean serum hormone concentrations +/- standard errors were as follows. In maternal peripheral blood up to 11 weeks 1.04 +/- 0.19 (LH-20) and 0.55 +/- 0.10 (HPLC), 12 to 23 weeks 1.22 +/- 0.23 (LH-20) and 0.67 +/- 0.77 (HPLC), 24 to 36 weeks 2.24 +/- 0.42 (LH-20) and 2.27 +/- 0.28 (HPLC), above 37 weeks 1.64 +/- 0.52 (LH-20) and 1.85 +/- 0.34 (HPLC), second stage of labor 5.07 +/- 1.28 (LH-20) and 4.73 +/- 0.54 (HPLC) ng/ml of steroid, respectively, were measured. Steroid concentration in umbilical arterial and venous blood were 7.87 +/- 1.41 (LH-20), 3.75 +/- 0.83 (HPLC) and 8.69 +/- 1.66 (LH-20), 5.38 +/- 0.87 (HPLC) ng/ml, respectively. The values of 16 alpha OH-A levels tended to increase as gestation proceeded. These data suggest production of 16 alpha OH-A by the feto-placental unit. An elevated level of 16 alpha OH-A is found in the umbilical venous blood as compared to the arterial samples. This indicates an extensive conversion. After onset of labor, the 16 alpha OH-A levels were significantly increased because of precursor steroid 16 alpha-hydroxydehydroepiandrosterone-sulfate is mobilized by the stress of labor.(ABSTRACT TRUNCATED AT 400 WORDS)

UI	MeSH Term	Description	Entries
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011261	Pregnancy Trimester, First	The beginning third of a human PREGNANCY, from the first day of the last normal menstrual period (MENSTRUATION) through the completion of 14 weeks (98 days) of gestation.	Early Placental Phase,Pregnancy, First Trimester,Trimester, First,Early Placental Phases,First Pregnancy Trimester,First Pregnancy Trimesters,First Trimester,First Trimester Pregnancies,First Trimester Pregnancy,First Trimesters,Phase, Early Placental,Phases, Early Placental,Placental Phase, Early,Placental Phases, Early,Pregnancies, First Trimester,Pregnancy Trimesters, First,Trimesters, First
D011262	Pregnancy Trimester, Second	The middle third of a human PREGNANCY, from the beginning of the 15th through the 28th completed week (99 to 196 days) of gestation.	Midtrimester,Pregnancy, Second Trimester,Trimester, Second,Midtrimesters,Pregnancies, Second Trimester,Pregnancy Trimesters, Second,Second Pregnancy Trimester,Second Pregnancy Trimesters,Second Trimester,Second Trimester Pregnancies,Second Trimester Pregnancy,Second Trimesters,Trimesters, Second
D011263	Pregnancy Trimester, Third	The last third of a human PREGNANCY, from the beginning of the 29th through the 42nd completed week (197 to 294 days) of gestation.	Pregnancy, Third Trimester,Trimester, Third,Last Trimester,Last Trimesters,Pregnancies, Third Trimester,Pregnancy Trimesters, Third,Third Pregnancy Trimester,Third Pregnancy Trimesters,Third Trimester,Third Trimester Pregnancies,Third Trimester Pregnancy,Third Trimesters,Trimester, Last,Trimesters, Last,Trimesters, Third
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005260	Female		Females
D005312	Fetal Blood	Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.	Cord Blood,Umbilical Cord Blood,Blood, Cord,Blood, Fetal,Blood, Umbilical Cord,Bloods, Cord,Bloods, Fetal,Bloods, Umbilical Cord,Cord Blood, Umbilical,Cord Bloods,Cord Bloods, Umbilical,Fetal Bloods,Umbilical Cord Bloods
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000735	Androstenedione	A delta-4 C19 steroid that is produced not only in the TESTIS, but also in the OVARY and the ADRENAL CORTEX. Depending on the tissue type, androstenedione can serve as a precursor to TESTOSTERONE as well as ESTRONE and ESTRADIOL.	4-Androstene-3,17-dione,delta-4-Androstenedione,4 Androstene 3,17 dione,delta 4 Androstenedione