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We purified acidic ribosomal proteins (P1 and P2) in good yield from rat liver ribosomes by precipitation of ribosomes with MgCl2 prior to ethanol extraction and chromatography of the extract on a column of CM-cellulose at pH 4.8. The newly-synthesized acidic ribosomal proteins in regenerating rat liver, labeled in vivo with [3H]leucine, were rapidly incorporated into cytoplasmic ribosomes without any detectable time lag and, after reaching a maximum at 30 min, they gradually disappeared from the ribosomes, suggesting a short metabolic-life. However, it was found later that they were re-incorporated slowly when newly-labeled proteins were "chased" by an injection of a large amount of cold leucine intraperitoneally at 15 min after the injection of [3H]leucine. Furthermore, in a long-term experiment, acidic ribosomal proteins were found to disappear with a half-life of 100 h from the ribosomes. Thus, these results suggest that acidic ribosomal proteins have a long metabolic life and are exchangeable on cytoplasmic ribosomes in regenerating rat liver.
K Tsurugi, and
K Ogata
January 1974,
Acta biologica et medica Germanica,
K Tsurugi, and
K Ogata
January 1983,
The International journal of biochemistry,
K Tsurugi, and
K Ogata
October 1972,
FEBS letters,
K Tsurugi, and
K Ogata
January 1987,
The International journal of biochemistry,
K Tsurugi, and
K Ogata
September 1973,
The Journal of biological chemistry,
K Tsurugi, and
K Ogata
January 1974,
Acta biologica et medica Germanica,
K Tsurugi, and
K Ogata
May 1976,
Journal of biochemistry,
K Tsurugi, and
K Ogata
November 1981,
Biochimica et biophysica acta,
K Tsurugi, and
K Ogata
April 1974,
Plant physiology,
K Tsurugi, and
K Ogata
October 1978,
Biochimica et biophysica acta,
