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A rapid method for auxotyping strains was developed that uses microtiter plates. This miniplate technique enables rapid identification of major auxotypes present in clinical strains. Additional growth requirements can be identified by adding individual amino acid supplements to complete gonococcal genetic medium. Analysis of 8 clones from 40 patients revealed that 10 had more than 1 auxotype. Deoxyribonucleic acid-mediated transformation can be used to establish whether the strains with apparently more than one auxotype are defective in the same locus in each of the involved biosynthetic pathways. Selection of more than one clone is required in precise epidemiological studies.
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
August 1987,
Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
March 2012,
Sexually transmitted diseases,
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
January 1981,
Acta dermato-venereologica,
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
September 1992,
The Indian journal of medical research,
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
August 1983,
Journal of medical microbiology,
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
January 1985,
Dermatologica,
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
February 1983,
The British journal of venereal diseases,
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
April 1983,
Schweizerische medizinische Wochenschrift,
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
April 1982,
The New England journal of medicine,
H B Short, and
V B Ploscowe, and
J A Weiss, and
F E Young
January 1982,
Bulletin de la Societe de pathologie exotique et de ses filiales,
