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The inactivation of thyrotrophin releasing hormone (pGlu-His-Pro-NH2, TRH) and its deamidated analogue pGlu-His-Pro-OH (TRH-OH) in human and rat serum has been studied using specific radioimmunoassays. No difference was apparent between human and rat serum with regard to proteolytic activity towards TRH and TRH-OH. It was found that the inactivation of both peptides is a saturable process. The disappearance of TRH was clearly inhibited by TRH-OH, luteinizing hormone-releasing hormone and dithiothreitol. The suppressive action of these compounds was observed to be dependent on their concentration. Proline and EDTA showed little inhibiting activity. Proline amide and pyroglutamic acid left the reaction unaffected. In no single instance could any production of TRH-OH from TRH be demonstrated.
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
February 1978,
The Journal of physiology,
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
July 1976,
Clinical endocrinology,
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
March 1976,
Molecular and cellular endocrinology,
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
September 1980,
The Journal of endocrinology,
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
November 1978,
The Journal of endocrinology,
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
January 1975,
Gerontologia clinica,
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
May 1975,
Clinical endocrinology,
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
June 1979,
Neuroscience letters,
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
April 1980,
Acta endocrinologica,
T J Visser, and
W Klootwijk, and
R Docter, and
G Hennemann
August 1987,
The Journal of endocrinology,
