BIOMAT Database Logo BIOMAT Database Logo

Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. 1977

K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer

A gene for somatostatin, a mammalian peptide (14 amino acid residues) hormone, was synthesized by chemical methods. This gene was fused to the Escherichia coli beta-galactosidase gene on the plasmid pBR322. Transformation of E. coli with the chimeric plasmid DNA led to the synthesis of a polypeptide including the sequence of amino acids corresponding to somatostatin. In vitro, active somatostatin was specifically cleaved from the large chimeric protein by treatment with cyanogen bromide. This represents the first synthesis of a functional polypeptide product from a gene of chemically synthesized origin.

UI MeSH Term Description Entries
D007786 Lactose Factors Plasmids which determine the ability of a bacterium to ferment lactose. Lac Factors,Factors, Lac,Factors, Lactose
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011498 Protein Precursors Precursors, Protein
D004274 DNA, Recombinant Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected. Genes, Spliced,Recombinant DNA,Spliced Gene,Recombinant DNA Research,Recombination Joint,DNA Research, Recombinant,Gene, Spliced,Joint, Recombination,Research, Recombinant DNA,Spliced Genes
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D001616 beta-Galactosidase A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1. Lactases,Dairyaid,Lactaid,Lactogest,Lactrase,beta-D-Galactosidase,beta-Galactosidase A1,beta-Galactosidase A2,beta-Galactosidase A3,beta-Galactosidases,lac Z Protein,Protein, lac Z,beta D Galactosidase,beta Galactosidase,beta Galactosidase A1,beta Galactosidase A2,beta Galactosidase A3,beta Galactosidases
D013004 Somatostatin A 14-amino acid peptide named for its ability to inhibit pituitary GROWTH HORMONE release, also called somatotropin release-inhibiting factor. It is expressed in the central and peripheral nervous systems, the gut, and other organs. SRIF can also inhibit the release of THYROID-STIMULATING HORMONE; PROLACTIN; INSULIN; and GLUCAGON besides acting as a neurotransmitter and neuromodulator. In a number of species including humans, there is an additional form of somatostatin, SRIF-28 with a 14-amino acid extension at the N-terminal. Cyclic Somatostatin,Somatostatin-14,Somatotropin Release-Inhibiting Hormone,SRIH-14,Somatofalk,Somatostatin, Cyclic,Somatotropin Release-Inhibiting Factor,Stilamin,Somatostatin 14,Somatotropin Release Inhibiting Factor,Somatotropin Release Inhibiting Hormone

Related Publications

K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. 1977.
January 1992, Biotechnology (Reading, Mass.),
K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
Expression in Escherichia coli of chemically synthesized gene for a human immune interferon.
January 1982, Nucleic acids symposium series,
K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
Expression in Escherichia coli of chemically synthesized gene for the human immune interferon.
March 1983, Nucleic acids research,
K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
Purification and characterization of the product of chemically synthesized human growth hormone gene expression in Escherichia coli.
October 1987, Chemical & pharmaceutical bulletin,
K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
High-level expression in Escherichia coli of a chemically synthesized gene for [Leu-28]echistatin.
June 1989, Gene,
K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
Expression in Escherichia coli of chemically synthesized gene for a novel opiate peptide alpha-neo-endorphin.
March 1982, Nucleic acids research,
K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
Partial purification and biological activity of the product of chemically synthesized human growth hormone gene expression in Escherichia coli.
August 1985, Chemical & pharmaceutical bulletin,
K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
Expression of the chemically synthesized gene for ribonuclease T1 in Escherichia coli using a secretion cloning vector.
May 1988, European journal of biochemistry,
K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
High-level expression of the chemically synthesized gene for microbial transglutaminase from Streptoverticillium in Escherichia coli.
May 1997, Bioscience, biotechnology, and biochemistry,
K Itakura, and T Hirose, and R Crea, and A D Riggs, and H L Heyneker, and F Bolivar, and H W Boyer
Production of biologically active N alpha-desacetylthymosin alpha 1 in Escherichia coli through expression of a chemically synthesized gene.
December 1980, Biochemistry,
Copied contents to your clipboard!