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An RNA-directed DNA polymerase was isolated from the peripheral blood leukocytes of a patient with acute myelomonocytic leukemia by successive purification of a particulate cytoplasmic fraction with endogenous, ribonuclease-sensitive DNA polymerase activity. Like RNA-directed DNA polymerase from mammalian type-C virus, the human leukemic cell enzyme efficiently utilized (A)(n).(dT)(12-18) and (C)(n).(dG)(12-18) and had an approximate molecular weight of 70,000. Further, the leukemic cell enzyme was strongly inhibited by antisera to RNA-directed DNA polymerase of primate type-C virus in a fashion similar to that noted with an extensively purified RNA-directed DNA polymerase from a person with acute myelogenous leukemia [Todaro, G.J. & Gallo, R.C. (1973), Nature 244, 206]. By these biochemical and immunological results the leukemic cell enzyme could be differentiated from all other known cellular DNA polymerases but could not be distinguished from RNA-directed DNA polymerase of primate type-C virus. We interpret these data, combined with observations published elsewhere, to indicate that human acute myelogenous leukemia cells contain components related to primate type-C virus. The parameters used in this study may provide the specificity and sensitivity required for determining the presence or absence and (if present) the relatedness of RNA-directed DNA polymerase in other cases and types of human leukemia.
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
May 1974,
Proceedings of the National Academy of Sciences of the United States of America,
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
September 1974,
European journal of biochemistry,
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
July 1973,
Nature,
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
March 1975,
Proceedings of the National Academy of Sciences of the United States of America,
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
January 1974,
Basic life sciences,
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
November 1971,
Nature,
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
January 1974,
Annals of clinical and laboratory science,
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
November 1973,
Proceedings of the National Academy of Sciences of the United States of America,
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
July 1977,
Blut,
R E Gallagher, and
G J Todaro, and
R G Smith, and
D M Livingston, and
R C Gallo
August 2006,
Journal of molecular biology,
