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A composite plasmid (pAT2010) has been constructed in vitro from RSF2124 and Bacillus subtilis IFO3022 plasmid (pAT1060) by covalent joining of the two DNA molecules by means of Escherichia coli DNA ligase through the cohesive ends generated by restriction endonuclease RI (EcoRI) cleavage. The composite plasmid was selected by transformation of E. coliC600r-m- with the ligated mixture after enrichment for composite plasmid by preparative agarose gel electrophoresis, and plating of the transformants on a medium containing ampicillin and colicin E1. Treatment of the composite plasmid with EcoRI yielded two fragments corresponding to the linear forms of the parental plasmids. The composite plasmids replicated as biologically functionally units in E. coli, and expressed genetic information carried by RSF2124. In the presence of chloramphenicol, the composite plasmids continued to replicate and the copy number gradually increased. Such nature of replication in the presence of chloramphenicol is characteristic to RSF2124 derived from colicin E1 factor, and so it is suggested that the replicator of RSF2124 is functional in the composite plasmid. The composite plasmid was found to synthesize mRNA of B. subtilis plasmid in cell-free extracts of E. Coli, by hybridization of the mRNA to the original plasmid DNA of pAT1060.
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
January 1982,
Acta microbiologica Polonica,
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
January 1991,
Research in microbiology,
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
December 1983,
Gene,
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
January 1984,
Molecular & general genetics : MGG,
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
January 1984,
Molecular & general genetics : MGG,
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
January 1984,
Molecular & general genetics : MGG,
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
November 1983,
Journal of bacteriology,
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
January 2005,
Molekuliarnaia biologiia,
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
January 1981,
Journal of bacteriology,
S Horinouchi, and
T Uozumi, and
T Hoshino, and
A Ozaki, and
S Nakajima, and
T Beppu, and
K Arima
August 1990,
FEMS microbiology letters,
