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The results of ribonuclease T1 oligonucleotide fingerprint analyses indicate that influenza virus messenger RNAs are incomplete transcripts of the corresponding genome RNAs and that in this respect they differ from the unpolyadenylated virus specific complementary RNAs obtained from infected cells. From the position of the untranscribed oligonucleotide in the virus RNA sequence and the ability or inability of the different transcripts to protect the 5' terminal nucleotide of virus RNAs against nuclease S1 digestion, it is concluded that whereas the unpolyadenylated cRNAs are complete transcripts, the polyadenylated messenger RNAs lack sequences complementary to the 5' end of the genome molecules.
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