BIOMAT Database Logo BIOMAT Database Logo

Membrane-bound nucleotidase of Bacillus cereus. 1978

W P Schrader, and J S Anderson

A membrane-bound nucleotidase of Bacillus cereus T was solubilized by digestion with trypsin and subsequently purified more than 300-fold. The purified nucleotidase was most active on ribonucleoside 5'-monophosphates and was slightly less active (40 to 60%) on deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates. In addition to hydrolytic activity, the nucleotidase preparation possessed phosphotransferase activity by which phosphate is transferred from a phosphate donor to the 5' position of nucleosides.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009708 Nucleotidases A class of enzymes that catalyze the conversion of a nucleotide and water to a nucleoside and orthophosphate. EC 3.1.3.-.
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D003854 Deoxyribonucleotides A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group. Deoxyribonucleotide
D001409 Bacillus cereus A species of rod-shaped bacteria that is a common soil saprophyte. Its spores are widespread and multiplication has been observed chiefly in foods. Contamination may lead to food poisoning.
D012265 Ribonucleotides Nucleotides in which the purine or pyrimidine base is combined with ribose. (Dorland, 28th ed) Ribonucleoside Phosphates,Ribonucleotide,Phosphates, Ribonucleoside
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities

Related Publications

W P Schrader, and J S Anderson
Membrane-bound 5'-nucleotidase/nucleoside phosphotransferase from Bacillus cereus.
November 1993, The International journal of biochemistry,
W P Schrader, and J S Anderson
Some properties of the membrane-bound penicillinase of Bacillus cereus 569.
January 1971, Acta microbiologica Academiae Scientiarum Hungaricae,
W P Schrader, and J S Anderson
The cell-bound penicillinase of Bacillus cereus.
August 1956, Journal of general microbiology,
W P Schrader, and J S Anderson
The succinate:menaquinone reductase of Bacillus cereus: characterization of the membrane-bound and purified enzyme.
June 2008, Canadian journal of microbiology,
W P Schrader, and J S Anderson
Isolation of a membrane-bound enzyme, 5'-nucleotidase.
January 1993, Methods in molecular biology (Clifton, N.J.),
W P Schrader, and J S Anderson
Characteristics of membrane-bound 5'-nucleotidase on human prostasomes.
July 1993, Clinica chimica acta; international journal of clinical chemistry,
W P Schrader, and J S Anderson
Enzymatic properties of the membrane-bound NADH oxidase system in the aerobic respiratory chain of Bacillus cereus.
November 2004, Journal of biochemistry and molecular biology,
W P Schrader, and J S Anderson
Properties of 5'-nucleotidase from Bacillus cereus obtained by washing intact cells with water.
August 1974, Biochemistry,
W P Schrader, and J S Anderson
Characterization of soluble vs membrane-bound human placental 5'-nucleotidase.
November 1990, Biochemical and biophysical research communications,
W P Schrader, and J S Anderson
Catalytic properties of Sepharose-bound L-alanine dehydrogenase from Bacillus cereus.
February 1983, Biochimica et biophysica acta,
Copied contents to your clipboard!