-transparent.png){kind=logo}
Liver homogenates have been submitted to quantitative fractionation by differential centrifugation. Three particulate fractions: N (nuclear), ML (large granules), and P (microsomes), and a final supernate (S) have been obtained. The biochemical composition of the microsomal fraction has been established from the assay and distribution pattern of 25 enzymatic and chemical constituents. These included marker enzymes for mitochondria (cytochrome oxidase), lysosomes (acid phosphatase and N-acetyl-beta-glucosaminidase), and peroxisomes (catalase). The microsomal preparations were characterized by a moderate contamination with large cytoplasmic granules (only 6.2% of microsomal protein) and by a high yield in microsomal components. Enzymes such as glucose 6-phosphatase, nucleoside diphosphatase, esterase, glucuronyltransferase, NADPH cytochrome c reductase, aminopyrine demethylase, and galactosyltransferase were recovered in the microsomes to the extent of 70% or more. Another typical behavior was shown by 5'-nucleotidase, alkaline phosphatase, alkaline phosphodiesterase I, and cholesterol, which exhibited a "nucleomicrosomal" distribution. Other complex distributions were obtained for several constituents recovered in significant amount in the microsomes and in the ML or in the S fraction.
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
April 1981,
The Journal of cell biology,
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
November 1976,
The Journal of cell biology,
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
November 1976,
The Journal of cell biology,
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
January 1986,
Advances in experimental medicine and biology,
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
March 1973,
The Journal of cell biology,
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
October 1978,
Biochimica et biophysica acta,
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
January 1985,
Folia biologica,
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
December 1971,
Biochimica et biophysica acta,
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
September 1968,
Life sciences,
A Amar-Costesec, and
H Beaufay, and
M Wibo, and
D Thinès-Sempoux, and
E Feytmans, and
M Robbi, and
J Berthet
August 2014,
Cold Spring Harbor protocols,
