Biomaterial Database

Continuous-flow automation of the Lactobacillus casei serum folate assay. 1977

G B Tennant

A method is described for the continuous-flow automation of the serum folate assay using Lactobacillus casei. The total incubation period is approximately four hours. The growth response of the organism to folate is estimated by measuring the rate of reduction of 2,3,5-triphenyl tetrazolium chloride (TTC). A simple continuous culture apparatus is used to grow the inoculum. Supplementation of the assay medium is necessary to obtain parallel results. A statistical assessment shows a favourable comparison with the whole-serum tube assay using a chloramphenicol resistant strain of L. casei. The method is less sensitive to inhibitory substances than the tube assay.

UI	MeSH Term	Description	Entries
D007780	Lacticaseibacillus casei	A rod-shaped bacterium isolated from milk and cheese, dairy products and dairy environments, sour dough, cow dung, silage, and human mouth, human intestinal contents and stools, and the human vagina. L. casei is CATALASE positive.	Lactobacillus casei
D005492	Folic Acid	A member of the vitamin B family that stimulates the hematopoietic system. It is present in the liver and kidney and is found in mushrooms, spinach, yeast, green leaves, and grasses (POACEAE). Folic acid is used in the treatment and prevention of folate deficiencies and megaloblastic anemia.	Pteroylglutamic Acid,Vitamin M,Folacin,Folate,Folic Acid, (D)-Isomer,Folic Acid, (DL)-Isomer,Folic Acid, Calcium Salt (1:1),Folic Acid, Monopotassium Salt,Folic Acid, Monosodium Salt,Folic Acid, Potassium Salt,Folic Acid, Sodium Salt,Folvite,Vitamin B9,B9, Vitamin
D001322	Autoanalysis	Method of analyzing chemicals using automation.	Autoanalyses
D001681	Biological Assay	A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.	Bioassay,Assay, Biological,Assays, Biological,Biologic Assay,Biologic Assays,Assay, Biologic,Assays, Biologic,Bioassays,Biological Assays
D013778	Tetrazolium Salts	Quaternary salts derived from tetrazoles. They are used in tests to distinguish between reducing sugars and simple aldehydes, for detection of dehydrogenase in tissues, cells, and bacteria, for determination of corticosteroids, and in color photography. (From Mall's Dictionary of Chemistry, 5th ed, p455)	Tetrazolium Salt,Salt, Tetrazolium,Salts, Tetrazolium
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor

Related Publications

G B Tennant

Modifications in the Lactobacillus casei assay of serum folate activity.

September 1966, American journal of clinical pathology,

G B Tennant

Modifications in the Lactobacillus casei assay of serum folate activity.

August 1966, Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists,

G B Tennant

Effect of serum-factors other than folate on the Lactobacillus casei assay.

January 1966, Lancet (London, England),

G B Tennant

Aseptic addition method for Lactobacillus casei assay of folate activity in human serum.

January 1966, Journal of clinical pathology,

G B Tennant

Effect of antimicrobial agents on the Lactobacillus casei folate assay.

October 1967, American journal of clinical pathology,

G B Tennant

The effect of different serum concentrations of antimicrobial agents on the Lactobacillus casei folate assay.

June 1974, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology,