Biomaterial Database

Modified culture technique for Corynebacterium diphtheriae isolation from desiccated swabs. 1978

R R Facklam, and D N Lawrence, and F O Sottnek

Corynebacterium diphtheriae was isolated from pyoderma and ulcerative skin lesions with a modified delayed culture procedure as late as 9 weeks after field collection of silica gel-desiccated swabs. Biotypes gravis and mitis were identified. Most isolates were nontoxigenic. Todd-Hewitt broth enrichment enhanced recovery of C. diphtheriae by 70%.

UI	MeSH Term	Description	Entries
D011711	Pyoderma	Any purulent skin disease (Dorland, 27th ed).	Pyodermas
D003353	Corynebacterium diphtheriae	A species of gram-positive, asporogenous bacteria in which three cultural types are recognized. These types (gravis, intermedius, and mitis) were originally given in accordance with the clinical severity of the cases from which the different strains were most frequently isolated. This species is the causative agent of DIPHTHERIA.
D003890	Desiccation	Removal of moisture from a substance (chemical, food, tissue, etc.).	Dessication
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D012867	Skin	The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D012883	Skin Ulcer	An ULCER of the skin and underlying tissues.	Skin Ulcers,Ulcer, Skin,Ulcers, Skin
D013020	South America	The southern continent of the Western Hemisphere, extending southward from the Colombia-Panama border.
D013048	Specimen Handling	Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.	Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings