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Cell-mediated immune responses in cattle naturally infected with strains of Brucella abortus and in cattle vaccinated with B abortus strain 19 during calfhood were studied by an in vitro lymphocyte-stimulation procedure. Lymphocytes were prepared from peripheral bovine blood by the Ficoll-diatrizoate technique, suspended in RPMI-1640 medium (1.5 X 10(6) lymphocytes/ml), cultured with B abortus-soluble antigen or phytohemagglutinin, and incubated for 6 days. Sixteen hours prior to termination of incubation, cultures were labeled with 1 muCi of [3H]thymidine (3HdT) and, after harvesting, assayed for 3HdT incorporation into DNA by liquid scintillation spectrometry. Lymphocytes from cattle with bacteriologically confirmed isolation of B abortus underwent a significantly higher lymphocyte stimulation with B abortus-soluble antigen than did cattle vaccinated with B abortus strain 19 during calfhood (P less than 0.005). Standard seroagglutination tests were conducted simultaneously with lymphocyte-stimulation tests, but there was no apparent correlation between levels of humoral antibodies and the cell-mediated immune responses as measured by in vitro specific lymphocyte stimulation.
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
August 1948,
The North American veterinarian,
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
March 1991,
Veterinary microbiology,
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
October 1966,
Canadian journal of comparative medicine and veterinary science,
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
June 1966,
Canadian journal of comparative medicine and veterinary science,
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
January 1989,
American journal of veterinary research,
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
January 1981,
The Veterinary record,
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
March 1973,
The Onderstepoort journal of veterinary research,
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
January 1979,
Journal of the American Veterinary Medical Association,
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
November 1981,
Research in veterinary science,
J M Kaneene, and
D W Johnson, and
R K Anderson, and
R D Angus, and
D E Pietz, and
C C Muscoplat
February 1995,
Veterinary immunology and immunopathology,
