-transparent.png){kind=logo}
A 48-hour radiometric test for determining the drug susceptibility of Mycobacterium tuberculosis has been developed. The test is based on the measurement of 14CO2 produced by the oxidation of formate labeled with carbon-14. The test system uses 5 X 10(7) organisms in 1 ml of Middlebrook 7H9 medium plus albumin-dextrose-catalase enrichment and 1 muCi of [14C]formate. The 14CO2 produced is measured in an ionization chamber at 24-, 48-, and 72-hour intervals, with and without the addition of antituberculous drugs. Isoniazid, streptomycin, rifampin, and ethambutol were each tested at 3 concentrations by the radiometric method and the reference (agar dilution) method. Six standard strains and 21 patient isolates were compared by both methods. Production of 14CO2 was quantitatively decreased in the presence of drugs that inhibit the organism. The radiometric method requires 2 days; the agar dilution, 14 to 21 days.
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
December 1984,
Zhonghua jie he he hu xi xi ji bing za zhi = Chinese journal of tuberculosis and respiratory diseases,
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
January 1989,
Research in microbiology,
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
October 1982,
The Journal of antimicrobial chemotherapy,
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
May 1981,
Journal of clinical microbiology,
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
November 1991,
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica,
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
December 1983,
Journal of clinical microbiology,
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
April 1995,
Journal of chemotherapy (Florence, Italy),
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
April 1981,
The American review of respiratory disease,
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
December 1986,
Antimicrobial agents and chemotherapy,
J A Kertcher, and
M F Chen, and
P Charache, and
C C Hwangbo, and
E E Camargo, and
P A McIntyre, and
H N Wagner
May 1988,
Diagnostic microbiology and infectious disease,
