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A clonal strain of epithelial cells (designated MH(1)C(1)) has been established from the transplantable Morris hepatoma No. 7795. The cells have maintained distinctive morphology throughout more than 20 subcultures (split 1:5) at 2- to 4-week intervals in supplemented Ham's F 10 medium. They contain many highly refractile, round, cytoplasmic bodies which stain bright red with Oil Red O. The population doubling time was 2 wk when the clonal strain was first established. It has gradually decreased to 1 wk. The cells synthesize rat serum albumin and secrete it into the culture medium as determined immunologically by microcomplement fixation and double diffusion. Albumin secretion (3-6 microg albumin/mg cell nitrogen/24 hr) occurs throughout the logarithmic phase of cell proliferation and has not diminished during serial propagation since the strain was initiated 15 months ago.
U I Richardson, and
A H Tashjian, and
L Levine
October 1977,
Gan,
U I Richardson, and
A H Tashjian, and
L Levine
December 1970,
The Journal of cell biology,
U I Richardson, and
A H Tashjian, and
L Levine
February 1968,
Endocrinology,
U I Richardson, and
A H Tashjian, and
L Levine
March 1971,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
U I Richardson, and
A H Tashjian, and
L Levine
June 1976,
Proceedings of the National Academy of Sciences of the United States of America,
U I Richardson, and
A H Tashjian, and
L Levine
March 1972,
Experimental cell research,
U I Richardson, and
A H Tashjian, and
L Levine
March 1973,
Experimental cell research,
U I Richardson, and
A H Tashjian, and
L Levine
January 1972,
Scandinavian journal of clinical and laboratory investigation,
U I Richardson, and
A H Tashjian, and
L Levine
March 1977,
Proceedings of the National Academy of Sciences of the United States of America,
U I Richardson, and
A H Tashjian, and
L Levine
November 1980,
In vitro,
