Biomaterial Database

Quantitative differentiation of Yersinia pestis strains by their murine toxin and fraction I contents. 1973

R P Tengerdy, and R P Hillam

Until now the serological typing of Yersinia pestis into subgroups has not proved possible because all the antigenic components are present in each isolate. Using acrylamide disk electrophoresis it was observed that differences exist in the protein distribution patterns of aqueous extracts from various Y. pestis isolates. One component that was especially plentiful in some Javanese and South American isolates was identified as the murine toxin. The amount of murine toxin varies significantly from isolate to isolate, and so permits them to be roughly grouped. In 28 degrees C cultures the variation in the murine toxin content is independent of the variation in the F-I content. A new method of typing based on this 2-trait variation in quantities, in contrast to classical typing based on qualitative differences, might prove to be a means of differentiating Y. pestis isolates.

UI	MeSH Term	Description	Entries
D007122	Immunoelectrophoresis	A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D004589	Electrophoresis, Disc	Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.	Electrophoresis, Disk,Disc Electrophoresis,Disk Electrophoresis
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000956	Antigens, Viral	Substances elaborated by viruses that have antigenic activity.	Viral Antigen,Viral Antigens,Antigen, Viral
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings
D014118	Toxins, Biological	Specific, characterizable, poisonous chemicals, often PROTEINS, with specific biological properties, including immunogenicity, produced by microbes, higher plants (PLANTS, TOXIC), or ANIMALS.	Biological Toxins
D015010	Yersinia pestis	The etiologic agent of PLAGUE in man, rats, ground squirrels, and other rodents.	Bacillus pestis,Bacterium pestis,Pasteurella pestis,Pestisella pestis,Yersinia pseudotuberculosis subsp. pestis
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus