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Three strains of Pseudomonas aeruginosa were demonstrated to transfer double-drug resistance by conjugation to a P. aeruginosa recipient at frequencies of 10(-4) to 10(-2) per recipient cell. Two of the three strains also transferred to Escherichia coli at frequencies which were 10(3)- to 10(5)-fold lower, but the third strain could not be demonstrated to do so. The latter strain, however, conferred maleness on the Pseudomonas recipient. The transfer of streptomycin resistance was associated with the acquisition of streptomycin phosphorylase by both P. aeruginosa and E. coli recipients. Maximal broth mating frequencies were obtained with nonagitated cultures less than 1 mm in depth. A pyocine selection system based on donor sensitivity and recipient resistance is described and appears to have future value as a generalized selective device for use after matings.
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
December 1987,
Bangladesh Medical Research Council bulletin,
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
February 1972,
Nature: New biology,
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
January 1991,
Antimicrobial agents and chemotherapy,
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
February 1973,
American journal of clinical pathology,
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
June 1974,
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde,
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
February 1993,
Journal of chemotherapy (Florence, Italy),
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
February 1973,
Lancet (London, England),
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
March 2014,
Central European journal of public health,
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
February 1979,
The Indian journal of medical research,
L E Bryan, and
H M Van Den Elzen, and
J T Tseng
January 1986,
Giornale di batteriologia, virologia ed immunologia,
