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A semi-automatized platelet counting procedure with the use of a Coulter Counter is described. Its originality consists in providing an electronic method of counting of the platelets after a differential centrifugation of the blood sample previously diluted with a hypertonic NaCl solution (density 1.053) which is isopycnic to platelet density. This procedure allows elimination of red and white cells without loss of platelets in the supernatant. The final platelet number can be calculated without further corrections. The method is quick and does not require specially skilled technicians. A single technician can perform 40 counts in one hour. The reproductibility of the method is excellent and the coefficient of variation 2,4%. The accuracy and feasibility of the method is also sustained by two years' experience in an haematological routine laboratory.
M de Bruyere, and
C Bellenot, and
G Sokal
January 1982,
Journal of clinical pathology,
M de Bruyere, and
C Bellenot, and
G Sokal
February 1995,
Biomaterials,
M de Bruyere, and
C Bellenot, and
G Sokal
January 1902,
Public health papers and reports,
M de Bruyere, and
C Bellenot, and
G Sokal
January 1980,
Preparative biochemistry,
M de Bruyere, and
C Bellenot, and
G Sokal
January 1978,
Acta biologica et medica Germanica,
M de Bruyere, and
C Bellenot, and
G Sokal
December 1971,
Scandinavian journal of clinical and laboratory investigation,
M de Bruyere, and
C Bellenot, and
G Sokal
March 1968,
Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists,
M de Bruyere, and
C Bellenot, and
G Sokal
March 1973,
Experimental cell research,
M de Bruyere, and
C Bellenot, and
G Sokal
October 1993,
Journal of clinical periodontology,
M de Bruyere, and
C Bellenot, and
G Sokal
January 1950,
Haematologica,
