BIOMAT Database Logo BIOMAT Database Logo

Defective prostaglandin synthesis by C3H/HeJ mouse macrophages stimulated with endotoxin preparations. 1979

L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen

Macrophages obtained from C3H/HeN mice produced significant amounts of prostaglandin E when exposed to phenol-extracted lipopolysaccharides (LPS), whereas macrophages from C3H/HeJ mice were unresponsive. The lipid A fraction from phenol-extracted LPS was an effective inducer or prostaglandin synthesis by macrophages from C3H/HeN mice. The polysaccharide portion of the LPS molecule had no effect. In contrast, the C3H/HeJ macrophages did not produce prostaglandin E in response to the lipid A moiety of phenol-extracted LPS. LPS prepared by butanol extraction stimulated the production of prostaglandin E by macrophages from both C3H/HeN and C3H/HeJ mice. The component of butanol-extracted LPS that stimulated the C3H/HeJ macrophages was shown to be a lipid A-associated protein. Further studies demonstrated a correlation between prostaglandin production by the macrophages of these two strains of mice in response to butanol- and phenol-extracted LPS and the lethal effects of the endotoxin preparations.

UI MeSH Term Description Entries
D008050 Lipid A Lipid A is the biologically active component of lipopolysaccharides. It shows strong endotoxic activity and exhibits immunogenic properties.
D008070 Lipopolysaccharides Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed) Lipopolysaccharide,Lipoglycans
D008264 Macrophages The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.) Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D011458 Prostaglandins E (11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities. PGE
D004731 Endotoxins Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells. Endotoxin
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Activation of C3H/HeJ macrophages by endotoxin.
November 1980, Journal of immunology (Baltimore, Md. : 1950),
L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Defective ceramide response in C3H/HeJ (Lpsd) macrophages.
September 1995, Journal of immunology (Baltimore, Md. : 1950),
L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Defective tumoricidal capacity of macrophages from C3H/HeJ mice.
January 1978, Journal of immunology (Baltimore, Md. : 1950),
L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Endotoxin-induced early gene expression in C3H/HeJ (Lpsd) macrophages.
September 1994, Journal of immunology (Baltimore, Md. : 1950),
L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Alveolar macrophages from C3H/HeJ mice show sensitivity to endotoxin.
May 1995, American journal of respiratory cell and molecular biology,
L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Endotoxin fails to induce procoagulant in C3H/HeJ exudate macrophages.
June 1983, Journal of the Reticuloendothelial Society,
L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Selection induction of prostaglandin E production in C3H/HeJ mouse macrophages by lipopolysaccharides from Bacteroides gingivalis.
December 1988, Oral microbiology and immunology,
L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Defective Fc-mediated phagocytosis in C3H/HeJ macrophages. II. Correction by cAMP agonists.
February 1981, Journal of immunology (Baltimore, Md. : 1950),
L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Misuse of the C3H/HeJ mouse in the determination of endotoxin contamination of various biologic preparations.
May 1978, Journal of immunology (Baltimore, Md. : 1950),
L M Wahl, and D L Rosenstreich, and L M Glode, and A L Sandberg, and S E Mergenhagen
Lps(d)/Ran of endotoxin-resistant C3H/HeJ mice is defective in mediating lipopolysaccharide endotoxin responses.
September 1999, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!