BIOMAT Database Logo BIOMAT Database Logo

Enzymatic dtermination of pyridoxal 5'-phosphate with gamma-cyanoaminobutyric acid aposynthase. 1979

K Abe, and C Ressler

A rapid alternative method is presented for the determination of pyridoxal 5'-phosphate (pyridoxal-P). The method involves the colorimetric analysis of thiocyanate liberated from S-cyanohomocysteine (Hcy (CN)) in the presence of cyanide when catalyzed by the pyridoxal-P dependent enzyme, gamma-cyano-alpha-aminobutyric acid (gamma-CNabu)-synthase (Hcy (CN) thiocyano-lyase [adding CN]). The rate of formation of thiocyanate is determined by the increase in absorbance at 470 nm on treatment of the enzymatic reaction mixture with FeCl3.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008190 Lyases A class of enzymes that catalyze the cleavage of C-C, C-O, and C-N, and other bonds by other means than by hydrolysis or oxidation. (Enzyme Nomenclature, 1992) EC 4. Desmolase,Desmolases,Lyase
D011732 Pyridoxal Phosphate This is the active form of VITAMIN B 6 serving as a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. During transamination of amino acids, pyridoxal phosphate is transiently converted into pyridoxamine phosphate (PYRIDOXAMINE). Pyridoxal 5-Phosphate,Pyridoxal-P,Phosphate, Pyridoxal,Pyridoxal 5 Phosphate,Pyridoxal P
D002151 Calorimetry The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D003486 Cyanides Inorganic salts of HYDROGEN CYANIDE containing the -CN radical. The concept also includes isocyanides. It is distinguished from NITRILES, which denotes organic compounds containing the -CN radical. Cyanide,Isocyanide,Isocyanides
D006710 Homocysteine A thiol-containing amino acid formed by a demethylation of METHIONINE. 2-amino-4-mercaptobutyric acid,Homocysteine, L-Isomer,2 amino 4 mercaptobutyric acid,Homocysteine, L Isomer,L-Isomer Homocysteine
D001051 Apoenzymes The protein components of enzyme complexes (HOLOENZYMES). An apoenzyme is the holoenzyme minus any cofactors (ENZYME COFACTORS) or prosthetic groups required for the enzymatic function. Apoenzyme
D001059 Apoproteins The protein components of a number of complexes, such as enzymes (APOENZYMES), ferritin (APOFERRITINS), or lipoproteins (APOLIPOPROTEINS). Apoprotein
D013861 Thiocyanates Organic derivatives of thiocyanic acid which contain the general formula R-SCN. Rhodanate,Rhodanates

Related Publications

K Abe, and C Ressler
Enzymatic fluorometric assay for plasma pyridoxal 5'-phosphate.
April 1991, Clinical biochemistry,
K Abe, and C Ressler
Stimulation of mouse brain gamma-hydroxybutyric acid transport by pyridoxal 5'-phosphate.
November 1994, Pharmacology,
K Abe, and C Ressler
Enzymatic activity of pyridoxine and pyridoxal-5-phosphate analogs.
January 1984, Progress in clinical and biological research,
K Abe, and C Ressler
[Interaction of pyridoxal-5'-phosphate and pyridoxamine-5'-phosphate with apo-gamma-aminobutyrate-glutamate transaminase].
September 1972, Doklady Akademii nauk SSSR,
K Abe, and C Ressler
Homogeneous, nonradioactive, enzymatic assay for plasma pyridoxal 5-phosphate.
September 2002, Clinical chemistry,
K Abe, and C Ressler
Inhibition of mouse brain synaptosomal gamma-aminobutyric acid transport by pyridoxal 5'-phosphate.
December 1980, Biochemical and biophysical research communications,
K Abe, and C Ressler
Interactions of L-glutamic acid, gamma-aminobutyric acid and Pyridoxal-5'-phosphate at ehe neuronal level.
January 1967, Schweizer Archiv fur Neurologie, Neurochirurgie und Psychiatrie = Archives suisses de neurologie, neurochirurgie et de psychiatrie,
K Abe, and C Ressler
Effects of the pyridoxal 5-phosphate analogue, pyridoxal 5-sulfate, on glutamic acid decarboxylase.
March 1961, Biochimica et biophysica acta,
K Abe, and C Ressler
Assay of pyridoxal-5'-phosphate, pyridoxal and pyridoxic acid in biological material.
January 1989, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition,
K Abe, and C Ressler
[Modification of the spectrophotometric method of determination of pyridoxal-5'-phosphate and pyridoxal in enzymatic preparations].
January 1976, Prikladnaia biokhimiia i mikrobiologiia,
Copied contents to your clipboard!